Analysis of genetic variation in selected generations of "Whole Red" pattern Cyprinus carpio var.color using ISSR markers

作者：Lv, Yao-Ping; Hu, Ze-Hui^*; Yang, Xiao-Qin; Wang, Cheng-Hui

来源：Biochemical Systematics and Ecology, 2012, 44: 243-249.

DOI：10.1016/j.bse.2012.05.002

• "Whole Red" pattern
• Cyprinus carpio var.color
• ISSR
• Genetic diversity
• Genetic variation

Inter Simple Sequence Repeats (ISSR) markers were applied to assess the genetic diversity in F-1, F-2, and F-3 generations of "Whole Red" pattern Cyprinus carpio.var.color as testing group, and their base population (F-0) as control group. Among four populations, 120, 118, 101 and 110 bands ranging from 200 to 2500 bp were amplified respectively. As analyzed, the percentage of polymorphic bands (PPB) ranged from 51.5% to 67.8%; Shannon's information index (H-0) varied from 0.2176 to 0.2745 among populations. With the increase of selected generations' populations, it showed a reduction in genetic diversity. Analysis of molecular variance revealed that a greater proportion of total genetic variation existed within populations (70%) rather than among populations (30%). Cluster analysis divided the four populations into two groups. Genetic difference value (G(st) from 0.1588 to 0.2766) indicated that the differentiation has taken place to some extent among "Whole Red" C carpio var.color.

• 出版日期2012-10
• 单位上海海洋大学; 浙江省海洋水产养殖研究所; 宁波大学; 丽水学院