Optimization of extraction of Tremella fuciformis polysaccharides and its antioxidant and antitumour activities in vitro

作者：Bin, Chen^*

来源：Carbohydrate Polymers, 2010, 81(2): 420-424.

DOI：10.1016/j.carbpol.2010.02.039

• Tremella fuciformis polysaccharides
• Response surface methodology
• Extraction
• Antioxidant
• Antitumour

Optimization of extraction of Tremella fuciformis polysaccharides was investigated using response surface methodology in this paper. T. fuciformis polysaccharides were extracted using boiling water at different extraction temperatures (80-100 degrees C), times (3.5-4.5 h) and ratios of solvent to raw material (4-6). The effect of extraction conditions on the yield of T. fuciformis polysaccharides were studied using a three-level three-factor Box-Behnken design. The results showed that the highest yield (3.08%) of T. fuciformis polysaccharides was reached at extraction temperature 100 degrees C, extraction time 4.5 h and ratio of solvent to raw material 5 (v/v). In addition, T. fuciformis polysaccharides could scavenge superoxide anion and hydroxyl radicals. At last, it could still be found that antitumour activities of T. fuciformis polysaccharides increased from 73.4% to 92.1% with increasing concentration of polysaccharides. Pharmacology experiment indicated that T. fuciformis polysaccharides was useful to the therapy of free radical injury and cancer diseases.

• 出版日期2010-6-11
• 单位上海交通大学