The synthesis, magnetic purification and evaluation of Tc-99m-labeled microbubbles

作者：Lazarova Neva; Causey Patrick W; Lemon Jennifer A; Czorny Shannon K; Forbes John R; Zlitni Aimen; Genady Afaf; Foster F Stuart; Valliant John F^*

来源：Nuclear Medicine and Biology, 2011, 38(8): 1111-1118.

DOI：10.1016/j.nucmedbio.2011.04.008

• Microbubbles
• Ultrasound
• Tc-99m
• SPECT

Introduction: Ultrasound (US) contrast agents based on micrbbubbles (MBs) are being investigated as platforms for drug and gene delivery. A methodology for determining the distribution and fate of modified MBs quantitatively in vivo can be achieved by tagging MBs directly with Tc-99m. This creates the opportunity to employ dual-modality imaging using both US and small animal SPECT along with quantitative ex vivo tissue counting to evaluate novel MB constructs.
Methods: A Tc-99m-labeled biotin derivative ((99m)TcL1) was prepared and incubated with streptavidin-coated MBs. The Tc-99m-labeled bubbles were isolated using a streptavidin-coated magnetic-bead purification strategy that did not disrupt the MBs. A small animal scintigraphic/CT imaging study as well as a quantitative biodistribution study was completed using (99m)TcL1 and Tc-99m-labeled bubbles in healthy C57B1-6 mice.
Results: The imaging and biodistribution data showed rapid accumulation and retention of Tc-99m-MBs in the liver (68.2 +/- 6.6 %ID/g at 4 min; 93.3 +/- 3.2 %ID/g at 60 min) and spleen (214.2 +/- 19.7 %ID/g at 4 min; 213.4 +/- 19.7 %1D/g at 60 min). In contrast, (99m)TcL1 accumulated in multiple organs including the small intestine (22.5 +/- 3.6 %ID/g at 4 min; 83.4 +/- 5.9 %ID/g at 60 min) and bladder (184.0 +/- 88.1 %ID/g at 4 min; 24.2 +/- 17.7 %ID/g at 60 min).
Conclusion: A convenient means to radiolabel and purify MBs was developed and the distribution of the labeled products determined. The result is a platform which can be used to assess the pharmacokinetics and fate of novel MB constructs both regionally using US and throughout the entire subject in a quantitative manner by employing small animal SPECT and tissue counting.

• 出版日期2011-11