Architecture of the Saccharomyces cerevisiae SAGA transcription coactivator complex

作者：Han Yan; Luo Jie; Ranish Jeffrey; Hahn Steven^*

来源：The EMBO Journal, 2014, 33(21): 2534-2546.

DOI：10.15252/embj.201488638

• coactivator
• gene regulation
• proteomics
• transcription

The conserved transcription coactivator SAGA is comprised of several modules that are involved in activator binding, TBP binding, histone acetylation (HAT) and deubiquitination (DUB). Crosslinking and mass spectrometry, together with genetic and biochemical analyses, were used to determine the molecular architecture of the SAGA-TBP complex. We find that the SAGA Taf and Taf-like subunits form a TFIID-like core complex at the center of SAGA that makes extensive interactions with all other SAGA modules. SAGA-TBP binding involves a network of interactions between subunits Spt3, Spt8, Spt20, and Spt7. The HAT and DUB modules are in close proximity, and the DUB module modestly stimulates HAT function. The large activator-binding subunit Tra1 primarily connects to the TFIID-like core via its FAT domain. These combined results were used to derive a model for the arrangement of the SAGA subunits and its interactions with TBP. Our results provide new insight into SAGA function in gene regulation, its structural similarity with TFIID, and functional interactions between the SAGA modules.
Synopsis
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The molecular interactions between SAGA subunits and its modules are determined using a combination of crosslinking and mass spectrometry together with genetic and biochemical analysis.
The TAF subunits of SAGA form a central core module, resembling that of TFIID. Spt7 and Spt20, two important structural subunits of SAGA, connect to distinct subunits and modules within the complex. The DUB and HAT modules are positioned close to each other within SAGA and functionally interact. Spt8 and Spt3 contact TBP at different locations and may simultaneously bind TBP. <doi origin="wiley" registered="yes">10.1002/(ISSN)1460-2075</doi

• 出版日期2014-11-3