A colorimetric assay of DNA methyltransferase activity based on peroxidase mimicking of DNA template Ag/Pt bimetallic nanoclusters

作者：Kermani Hanie Ahmadzade; Hosseini Morteza^*; Miti Andrea; Dadmehr Mehdi; Zuccheri Giampaolo; Hosseinkhani Saman; Ganjali Mohammad Reza

来源：Analytical and Bioanalytical Chemistry, 2018, 410(20): 4943-4952.

DOI：10.1007/s00216-018-1143-2

• Ag/Pt nanoclusters
• DNA methyltransferase
• Colorimetric detection
• Enzyme mimic

DNA methylation catalyzed by DNA methyl transferase (MTase) is a significant epigenetic process for modulating gene expression. Abnormal levels of DNA MTase enzyme have been regarded as a cancer biomarker or a sign of bacterial diseases. We developed a novel colorimetric method to assay M. SssI MTase activity employing peroxidase-like activity of DNA template Ag/Pt NCs without using restriction enzymes. Based on inhibiting the peroxidase reaction that occurred in the TMB-H2O2 system, in the presence of MTase, a highly sensitive and selective colorimetric biosensor was fabricated with a detection limit (LOD) of 0.05 U/mL and a linear range from 0.5 to 10 U/mL. The changes in absorption intensity were monitored to quantify the M. SssI activity. This strategy had a high selectivity over other proteins. Furthermore, it is also demonstrated that this method can be used for the evaluation and screening of inhibitors for DNA MTase.

• 出版日期2018-8