Detection of antibiotic-resistant bacteria endowed with antimicrobial activity from a freshwater lake and their phylogenetic affiliation

作者：Zothanpuia; Passari Ajit K; Gupta Vijai K; Singh Bhim P^*

来源：PeerJ, 2016, 4: e2103.

DOI：10.7717/peerj.2103

• BOX-PCR
• Antibiotic susceptibility
• PKS II
• ERIC-PCR
• NRPS
• 16S rRNA gene

Antimicrobial resistance poses a serious challenge to global public health. In this study, fifty bacterial strains were isolated from the sediments of a freshwater lake and were screened for antibiotic resistance. Out of fifty isolates, thirty-three isolates. showed resistance against at least two of the selected antibiotics. Analysis of 16S rDNA sequencing revealed that the isolates belonged to ten different genera, namely Staphylococcus (n = 8), Bacillus (n = 7), Lysinibacillus (n = 4), Achromobacter (n=3), bacterium (n = 3), Methylobacterium (n = 2), Bosea (n = 2), Aneurinibacillus (n = 2), Azospirillum (n = 1), Novosphingobium (n = 1). Enterobacterial repetitive intergenic consensus (ERIC) and BOX-PCR markers were used to study the genetic relatedness among the antibiotic resistant isolates. Further, the isolates were screened for their antimicrobial activity against bacterial pathogens viz., Staphylococcus aureus (MTCC-96), Pseudomonasl aeruginosa (MTCC-2453) and Escherichia coli (MTCC-739), and pathogenic fungi viz., Fusarium proliferatum (MTCC-286), Fusarium oxysporum (CABI-293942) and Fusarium oxy. ciceri (MTCC-2791). In addition, biosynthetic genes (polyketide synthase II (PKS-II) and non-ribosomal peptide synthetase (NRPS)) were detected in six and seven isolates, respectively. This is the first report for the multifunctional analysis of the bacterial isolates from a wetland with biosynthetic potential, which could serve as potential source of useful biologically active metabolites.

• 出版日期2016-6-9