Development of Vitamin D Determination in Infant Formula by Column-Switching HPLC with UV Detector

作者：Ko Jinhyouk; Kwak Byung Man; Ahn Jang Hyuk^*; Shim Sung Lye; Kim Kyong Su; Yoon Taehyung; Leem Dong gil; Jeong Jayoung

来源：Korean Journal for Food Science of Animal Resources, 2012, 32(5): 571-577.

DOI：10.5851/kosfa.2012.32.5.571

• vitamin D
• column-switching high-performance liquid chromatography
• UV detection
• infant formula

This study was carried out to develop an analytical method for the determination of vitamin D in infant formula. Vitamin D was determined by column-switching high-performance liquid chromatography (HPLC) equipped with a reversed phase column and UV detector after saponification and extraction of the formula with an organic solvent. A pre-separation column (C-8) focusing column (C-18), analytical column (C-18) and UV-Vis detector (254 nm) were used. The limits of detection (LOD) and the limits of quantification (LOQ) for vitamin D were estimated to be 1.51 mu g/kg and 4.95 mu g/kg, respectively. The linearity, recovery, precision and accuracy of the analytical method for vitamin D were evaluated through the application of a SRM (Standard Reference Material) 1846 (National Institute of Standard %26 Technology, USA). The linearity of this method was calculated with a value of the coefficient of determination (r(2))%26gt;= 0.9999. The recovery of vitamin D was 85.20 +/- 3.00%. The intra-assay precision for vitamin D was between 1.68 +/- 0.03% and 5.75 +/- 0.33%, and the inter-assay precision for vitamin D ranged from 1.73 +/- 0.03% to 2.96 +/- 0.09%. The intra-assay accuracy for vitamin D was between 100.03 +/- 2.77% and 102.01 +/- 0.59%, and the inter-assay accuracy for vitamin D ranged from 99.00 +/- 1.53% to 102.01 +/- 3.04%. The proposed method is optimal for the separation and quantification of vitamin D from infant formula.

• 出版日期2012-10