A novel human KRAB-containing zinc-finger gene ZNF446 inhibits transcriptional activities of SRE and AP-1

作者：Liu, F; Zhu, CB; Xiao, J; Wang, YQ; Tang, WX; Yuan, WZ; Zhao, YL; Li, YQ; Xiang, ZM; Wu, XS; Liu, MY^*

来源：Biochemical and Biophysical Research Communications, 2005, 333(1): 5-13.

DOI：10.1016/j.bbrc.2005.05.069

• ZNF446
• Kruppel type zinc finger
• KRAB
• transcriptional repressor
• heart development
• SRF and AP-1
• MAPK signaling pathway

Kruppel-related zinc-finger proteins constitute the largest individual family of transcription factors in mammals [C. Looman, L. Hellman, M. Abrink, A novel Kruppel-associated box identified in a panel of mammalian zinc-finger proteins, Mammalian Genome 15 (I) (2004) 35-40. [1]]. Here we identified and characterized a novel zinc-finger gene named ZNF446. The predicted protein contains a KRAB and three C2H2 zinc fingers. Northern blot analysis shows that ZNF446 is expressed in a variety of human adult tissues with the highest expression level in muscle. ZNF446 is a transcription repressor when fused to GAL4 DNA-binding domain and co-transfected with VP-16. Overexpression of ZNF446 in COS-7 cells inhibits the transcriptional activities of SRE and AP-1, in which the KRAB motif represents the basal transcriptional repressive activity, suggesting that the ZNF446 protein may act as a transcriptional repressor in mitogen-activated protein kinase (MAPK) signaling pathway to mediate cellular functions.

• 出版日期2005-7-22
• 单位湖南师范大学