A New Ethylene-Responsive Factor CaPTI1 Gene of Pepper (Capsicum annuum L.) Involved in the Regulation of Defense Response to Phytophthora capsici

作者：Jin, Jing-Hao; Zhang, Huai-Xia; Tan, Jun-Yi; Yan, Ming-Jia; Li, Da-Wei; Khan, Abid; Gong, Zhen-Hui^*

来源：Frontiers in Plant Science, 2016, 6: 1217.

DOI：10.3382/fpis.2015.01217

• pepper
• ethylene-responsive factor (ERF)
• Phytophtora capsici
• relative expression
• virus-induce gene silencing (VIGS)

Ethylene-responsive factors (ERF) are usually considered to play diverse roles in plant response to biotic and abiotic stresses. In this study, an ERF gene CaPT11 was isolated from pepper transcriptome database. CaPT11 contains an open reading frame (ORF) of 543 bp, which encodes a putative polypeptide of 180 amino acids with a theoretical molecular weight of 2030. kDa. Results of expression profile showed that CaPT11 had a highest expression level in roots and this gene could not only response to the infection of Phytophthora capsici and the stresses of cold and drought, but also be induced by the signaling molecule (salicylic acid, Methyl Jasmonate, Fthephon, and hydogen peroxide). Furthermore, virus induce gene silencing (VIGS) of CaPT11 in pepper weakened the defense response significantly by reducing the expression of defense related genes CaPFR1, CaDEF1 and CaSAR82 and also the root activity. These results suggested that CaPT11 is involved in the regulation of defense response to P. capsici in pepper.

• 出版日期2016-1-8
• 单位西北农林科技大学