Molecular detection of bovine herpesvirus 1 and 5 in formalin-fixed, paraffin-embedded samples from cattle with neurological disease

作者：Arruda Laura P^*; Nakazato Luciano; Dutra Valeria; Lemos Ricardo A A; Nogueira Ana P A; Cruz Raquel A S; Pescador Caroline A; Colodel Edson M

来源：Pesquisa Veterinaria Brasileira, 2010, 30(8).

• Bovine herpesvirus
• BoHV-1
• BoHV-5
• PCR

Arruda L. P., Nakazato L., Dutra V., Lemos R. A. A., Nogueira A. P. A., Cruz R. A. S., Pescador C. A. & Colodel E. M. 2010. [Molecular detection of bovine herpesvirus 1 and 5 in formalin-fixed, paraffin-embedded samples from cattle with neurological disease.] Deteccao molecular de herpesvirus bovino 1 e 5 em amostras de encefalo conservadas em formol e emblocadas em parafina provenientes de bovinos com doenca neurologica. Pesquisa Veterinaria Brasileira 30(8): 646-650. Departamento de Clinica Medica Veterinaria, Faculdade de Agronomia e Medicina Veterinaria, Universidade Federal de Mato Grosso, Av. Fernando Correa da Costa s/n, Bairro Coxipo, Cuiaba, MT 78068-900, Brazil. E-mail: [e-mail]
Bovine herpesvirus (BoHV) is an important cause of neurological disease in cattle in the Midwest Brazil. The application of molecular diagnostic techniques represents an important contribution for the study of BoHV. This paper describes the detection of BoHV-5 and BoHV-1 by a specific multiplex PCR assay in 76 paraffin-embedded samples from central nervous system (CNS) of cattle with neurological disorders. The samples were divided into 2 groups according to the histological features: Group 1 was composed of 40 cases of necrotizing meningoencephalitis (characteristic of BoHV infection), and Group 2 was composed of 36 cases of nonspecific nonsuppurative meningoencephalitis. Positive results for BoHV-5 accounted for 40% of the samples in the group 1 and 33% in the group 2. No detection of BoHV-1 was recorded.

• 出版日期2010-8