A Color-coded Imaging Model of the Interaction of alpha(v) Integrin-GFP Expressed in Osteosarcoma Cells and RFP Expressing Blood Vessels in Gelfoam (R) Vascularized In Vivo

作者：Uehara Fuminari; Tome Yasunori; Yano Shuya; Miwa Shinji; Mii Sumiyuki; Hiroshima Yukihiko; Bouvet Michael; Maehara Hiroki; Kanaya Fuminori; Hoffman Robert M^*

来源：Anticancer Research, 2013, 33(4): 1361-1366.

• alpha(v) Integrin
• green fluorescent protein
• blood vessels
• red fluorescent protein
• Gelfoam (R)
• osteosarcoma
• transgenic nude mouse
• confocal microscopy

The integrin family of proteins has been shown to be involved in the malignant behavior of cells. We report here development of a color-coded imaging model that can visualize the interaction between a integrin linked to green fluorescent protein (GFP) in osteosarcoma cells and blood vessels in Gelfoam (R) vascularized after implantation in red fluorescent protein (RFP) transgenic nude mice. Human 143B osteosarcoma cells expressing a(v) integrin-GFP were generated by transfection with an a(v) integrin-GFP vector. Gelfoam (R) (5x5 mm) was transplanted subcutaneously in transgenic RFP nude mice. The implanted Gelfoam (R) became highly vascularized with RFP vessels within 14 days. Skin flaps were made at days 7, 14, 21, 28 after transplantation of Gelfoam for observing vascularization of the Gelfoam (R) using fluorescence imaging. Gelfoam (R) is a useful tool to observe angiogenesis in vivo. 143B cells (5x10(5)) expressing a(v) integrin-GFP were injected into the Gelfoam (R) seven days after transplantation of Gelfoam (R). Seven days after cancer-cell injection, cancer cells and blood vessels were observed in the Gelfoam (R) by color-coded confocal microscopy via the skin flap. The 143B cells expressing a(v) integrin-GFP proliferated into the Gelfoam (R), which contained RFP-expressing blood vessels. Strong expression of a(v) integrin-GFP in 143B cells was observed near RFP vessels in the Gelfoam (R). The observation of the behavior of a(v) integrin-GFP and blood vessels will allow further understanding of the role of a(v) integrin in cancer cells.

• 出版日期2013-4