Autoinhibition of IL-15 expression in KC cells is ERK1/2 and PI3K dependent

作者：Zhang, S. -Q.; Luo, X.; Yang, S.; Liu, J. -L.; Yang, C. -J.; Yin, X. -Y.; Huang, H. -L.; Zhang, X. -J.^*

来源：Scandinavian Journal of Immunology, 2008, 68(4): 397-404.

DOI：10.1111/j.1365-3083.2008.02150.x

• NF-KAPPA-B
• GROWTH-FACTOR
• SCID MOUSE
• INTERLEUKIN-15
• PSORIASIS
• KERATINOCYTE
• ACTIVATION
• CYTOKINE
• IDENTIFICATION
• SECRETION

Interleukin (IL)-15 is a proinflammatory cytokine and plays a key role in many diseases, including psoriasis. Although its signal transduction pathways in keratinocytes (KC) have been partially elucidated, the effects of IL-15 on expression of IL-15, IL-6 and TNF-alpha in KC are unknown. We have investigated the effects of IL-15 on the expression of the three genes in primary culture of KC by the real-time PCR, Western blot and ELISA. We observed that exogenous IL-15 suppressed the endogenous expression of IL-15, decreased the expression of IL-6 at mRNA and protein levels in KC. The inhibition was blocked by anti-IL-15 monoclonal antibody and by inactive IL-15, I50D mutant IL-15. In contrast, IL-15 increased TNF-alpha transcription in these cells. Mechanistic studies demonstrated that the auto-regulation of IL-15 expression was dependent on activity of ERK1/2 and PI3K. Our studies suggest that there is an auto-inhibitory mechanism controlling cellular IL-15 levels.

• 出版日期2008-10
• 单位安徽医科大学