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摘要
The study was conducted to determine the antibiotic resistance and virulence factors profile of Escherichia coli isolated from chicken faeces sample. Differential and selective media were used to isolate a total of 164 E. coli strains from 180 faeces samples at different chicken farm in east China. These strains were tested against 15 antibiotics using the agar disc diffusion. The isolates showed high resistance rates to sulfamethoxazole (91.5%), nalidixic acid (86%), tetracycline (85.4%), streptomycin (80.5%), ampicillin (79.9%), Trimethoprim-sulfamethoxazole (74.4%), Enrofloxacin (67.1%), Ciprofloxacin (56.7%), Norfloxacin (54.9%) and Chloramphenicol (51.2%). Rates of resistance to Amoxicillin/clavulanic acid, Gentamicin and Florfenicol were moderate (36, 37.8 and 14.6%), while low rates were recorded for Amikacin (9.2%) and Cefalexin (5.5%). Among the 164 isolates, 36 (23.7%) were haemolytic, 42 (27.6%) were hydrophobic, 132 (86.8%) were serum resistant and only four were positive for protease. Eleven virulence-associated genes, including iucA, tsh, Iss, fimH, sitA, hlyF, iron, traT, cdtB, ibeA, and cvaC, were screened for via polymerase chain reaction (PCR) amplification. The fimH gene was the most prevalent with a detection rate of 86.6%, followed by traT (58.5%), iron (29.3%), and Iss (25%) in these avian isolates.
- 出版日期2012-2-23
- 单位中国药科大学
