Novel HIT antibody detection method using Sonoclot (R) coagulation analyzer

作者：Wanaka Keiko^*; Asada Reiko; Miyashita Kumiko; Kaneko Makoto; Endo Hirokazu; Yatomi Yutaka

来源：Thrombosis Research, 2015, 135(1): 127-129.

DOI：10.1016/j.thromres.2014.10.024

• Heparin induced thrombocytopenia
• Blood coagulation activity
• Sonoclot
• Monoclonal antibody

Introduction: Since heparin-induced thrombocytopenia (HIT), caused by the generation of antibodies against platelet factor 4 (PF4)/heparin complexes (HIT antibodies), may induce serious complications due to thrombosis, a prompt diagnosis is desirable. Functional tests with platelet activation to detect HIT antibodies are useful for diagnosis of HIT, in particular C-14-selotonin release assay (SRA). However, they are complicated and so can be performed only in limited laboratories. We tested if a blood coagulation test using Sonoclot (R) analyzer can serve for the detection of HIT antibodies.
Materials and Methods: A murine monoclonal antibody (HIT-MoAb) against PF4/heparin complexes was used as an alternative to human HIT antibodies. To themixture of HIT-MoAb and heparin (0.5 U/mL, final), whole blood obtained from a healthy volunteer was added, and then the activated clotting time (ACT), clot rate (CR), and area under the curve (AUC) were measured with Sonoclot (R) analyzer for 30 minutes.
Results: The HIT-MoAb (30 to 100 mu g/mL, final) concentration dependently suppressed the anticoagulation activity (prolongation of ACT and decrease of CR and AUC) of heparin.
Conclusions: The suppression of anticoagulation effect of heparin by HIT-MoAb was demonstrated by measurements using Sonoclot (R) analyzer. This method may provide a new tool for screening of HIT antibodies.

• 出版日期2015-1