Unambiguous structural elucidation of base-modified purine nucleosides using NMR

作者：Narukulla Raman; Shuker David E G; Ramesh Vasudevan; Xu Yao Zhong^*

来源：Magnetic Resonance in Chemistry, 2008, 46(1): 1-8.

DOI：10.1002/mrc.2111

• NMR
• H-1
• C-13
• N-15
• purine
• modified purine
• base
• nucleoside
• DNA

A general and unambiguous approach has been developed for structural elucidation of modified purine nucleosides using NMR spectroscopy. Systematic assignment of proton and carbon signals of modified nucleosides was firmly established by COSY and the anomerism of the glycosidic linkage of synthetic nucleosides clearly elucidated by NOESY experiments. Characteristic properties of N-15-isotopic labelling at specific positions of nucleosides were also employed for structural studies. The reported approach is applicable to other modified nucleosides and nucleotides, as well as nucleobases.

• 出版日期2008-1