Altered intracellular signaling cascades in peripheral blood mononuclear cells from BD patients

作者：Barbosa Izabela Guimaraes^*; Nogueira Camila R C; Rocha Natalia Pessoa; Lemos Queiroz Ana Luiza; Vago Juliana Priscila; Tavares Luciana Padua; Assis Frankcineia; Fagundes Caio Tavares; Huguet Rodrigo Barreto; Bauer Moises Evandro; Teixeira Antonio Lucio; de Sousa Lirlandia Pires

来源：Journal of Psychiatric Research, 2013, 47(12): 1949-1954.

DOI：10.1016/j.jpsychires.2013.08.019

• Bipolar disorder
• Immunology
• Inflammation
• MAPK
• Mania
• Nuclear factor kappa B

Bipolar disorder (BD) is a severe psychiatric disorder of complex physiopathology that has been associated with a pro-inflammatory state. The aim of the present study was to investigate intracellular pathways associated with inflammatory signaling, assessing the phosphorylation levels of transcription factor nuclear factor kappa B (NF-kappa B) and mitogen-activated protein kinase (MAPKs) in peripheral blood mononuclear cells of euthymic BD patients and healthy controls. Fifteen BD euthymic type I patients, and 12 healthy controls matched by age and gender were enrolled in this study. All subjects were assessed by the Mini-International Neuropsychiatry Interview and the patients also by the Young Mania Rating Scale and the Hamilton Depression Rating Scale. Phosphorylation levels of p65 NF-kappa B subunit, and MAPK ERK1/2, and p38 were assessed by Western blot and flow cytometry. Plasma cytokines (IL-2, IL-4, IL6, IL-10, IFN-gamma, TNF-alpha, and IL-17A) were measured using cytometric bead arrays. Western blot and flow cytometry analyses showed increased phosphorylation levels of p65 NF-kappa B subunit, and MAPKs ERK1/2, and p38 in BD patients in euthymia in comparison with controls. BD patients presented increased proinflammatory cytokines levels in comparison with controls, and TNF-alpha correlated with the levels of phosphorylated p65 NF-kappa B. The present study found increased activation of MAPK and NF-kappa B pathways in BD patients, which is in line with a pro-inflammatory status.

• 出版日期2013-12