Dynamic 3D cell culture via a chemoselective photoactuated ligand

作者:Westcott Nathan P; Luo Wei; Goldstein Jeffrey; Yousaf Muhammad N*
来源:Biointerphases: An Open Access Journal for the Biomaterials Interface Community , 2014, 9(3): 031005.
DOI:10.1116/1.4881035

摘要

A new strategy to create a dynamic scaffold for three-dimensional (3D) cell experiments based on a photo-activated cell adhesive peptide ligand is described. After polymerization, the inert matrix becomes cell adhesive by chemoselective modification through the conjugation of oxyamine-terminated ligands. Furthermore, spatial and temporal control of cell culture within the 3D matrix was achieved by the use of a biospecific photoprotected peptide and visualized by confocal microscopy.

  • 出版日期2014-9