Determination of Vanillin in Milk Powder by Capillary Electrophoresis Combined with Dispersive Liquid-Liquid Microextraction

作者：Shu, Ming; Man, Yanru; Ma, Hui; Luan, Feng; Liu, Huitao^*; Gao, Yuan^*

来源：Food Analytical Methods, 2016, 9(6): 1706-1712.

DOI：10.1007/s12161-015-0347-8

• Capillary zone electrophoresis (CZE)
• Dispersive liquid-liquid microextraction (DLLME)
• Milk powder
• Vanillin

A simple method using dispersive liquid-liquid microextraction (DLLME) combined with capillary zone electrophoresis (CZE) was developed for detection and quantification of vanillin in milk powder. CZE analytical conditions and DLLME parameters were optimized. The optimal running buffer was 10 mM borate buffer at pH 8.0. 4-Hydroxybenzaldehyde was used as the internal standard. The optimized microextraction conditions were 5.0 mL sample solution at pH 5.5, 8 % (m/v) NaCl added, with 120 mu L CHCl3 (extraction solvent), and 0.8 mL tetrahydrofuran (THF) (disperser solvent) injected. Under the optimum extraction and detection conditions, vanillin was determined in 5 min, with the limit of detection at 0.02 mu g mL(-1) and a quantitation range of 0.05-10 mu g mL(-1). The method was successfully applied to the analysis of vanillin in infant milk powder samples. The recoveries obtained were between 89.7 and 95.1 % with relative standard deviation (RSD) less than 2.5 %.

• 出版日期2016-6
• 单位烟台大学