A novel amperometric biosensor based on ZnO nanoparticles-modified carbon paste electrode for determination of glucose in human serum

作者：Aydogdu Gozde^*; Zeybek Derya Koyuncu; Pekyardimci Sule; Kilic Esma

来源：Artificial Cells, Nanomedicine and Biotechnology, 2013, 41(5): 332-338.

DOI：10.3109/21691401.2012.744994

• amperometric biosensor
• carbon paste electrode
• glucose
• human serum
• ZnO nanoparticles

Zinc oxide nanoparticles-(ZnONPs) modified carbon paste enzyme electrodes (ZnONPsMCPE) were developed for determination of glucose. The determination of glucose was carried out by oxidation of H2O2 at +0.4 V. ZnONPsMCPE provided biocompatible microenvironment for GOx and necessary pathway of electron transfer between GOx and electrode. The response of GOx/ZnONPsMCPE was proportional to glucose concentration and detection limit was 9.1 X 10(-3) mM. K-m and I-max, were calculated as 0.124 mM and 2.033 mu A. The developed biosensor exhibits high analytical performance with wide linear range (9.1. X 10(-3) -14.5 mM), selectivity and reproducibility. Serum glucose results allow us to ascertain practical utility of GOx/ZnONPsMCPE biosensor.

• 出版日期2013-10