Distinguishing N-acetylneuraminic acid linkage isomers on glycopeptides by ion mobility-mass spectrometry

作者：Hinneburg H; Hofmann J; Struwe W B; Thader A; Altmann F; Silva D Varon; Seeberger P H; Pagel K^*; Kolarich D^*

来源：Chemical communications, 2016, 52(23): 4381-4384.

DOI：10.1039/c6cc01114d

Differentiating the structure of isobaric glycopeptides represents a major challenge for mass spectrometry-based characterisation techniques. Here we show that the regiochemistry of the most common N-acetylneuraminic acid linkages of N-glycans can be identified in a site-specific manner from individual glycopeptides using ion mobility-mass spectrometry analysis of diagnostic fragment ions.

• 出版日期2016