Nitric oxide prevents axonal degeneration by inducing HIF-1-dependent expression of erythropoietin

作者：Keswani Sanjay C; Bosch Marce Marta; Reed Nicole; Fischer Angela; Semenza Gregg L^*; Hoeke Ahmet

来源：Proceedings of the National Academy of Sciences of the United States of America, 2011, 108(12): 4986-4990.

DOI：10.1073/pnas.1019591108

Nitric oxide (NO) is a signaling molecule that can trigger adaptive (physiological) or maladaptive (pathological) responses to stress stimuli in a context-dependent manner. We have previously reported that NO may signal axonal injury to neighboring glial cells. In this study, we show that mice deficient in neuronal nitric oxide synthase (nNOS(-/-)) are more vulnerable than WT mice to toxin-induced peripheral neuropathy. The administration of NO donors to primary dorsal root ganglion cultures prevents axonal degeneration induced by acrylamide in a dose-dependent manner. We demonstrate that NO-induced axonal protection is dependent on hypoxia-inducible factor (HIF)-1-mediated transcription of erythropoietin (EPO) within glial (Schwann) cells present in the cultures. Transduction of Schwann cells with adenovirus AdCA5 encoding a constitutively active form of HIF-1 alpha results in amelioration of acrylamide-induced axonal degeneration in an EPO-dependent manner. Mice that are partially deficient in HIF-1 alpha (HIF-1 alpha(+/-)) are also more susceptible than WT littermates to toxic neuropathy. Our results indicate that NO -> HIF-1 -> EPO signaling represents an adaptive mechanism that protects against axonal degeneration.

• 出版日期2011-3-22