Anti-ulcerogenic effect of Zuojin Pill against ethanol-induced acute gastric lesion in animal models

作者：Wang, Jing; Zhang, Tianzhu; Zhu, Lingpeng; Ma, Chunhua^*; Wang, Shumin

来源：Journal of Ethnopharmacology, 2015, 173: 459-467.

DOI：10.1016/j.jep.2015.04.017

• Zuojin Pill
• Coptis chinensis Franch
• Tetradium ruticarpum (A. Juss.) Hartley
• Gastric ulcer
• NF-kappa B pathway

Ethnopharmacological relevance: Zuojin Pill (ZJP), a traditional Chinese medicinal decoction, contains two herbal drugs: Coptis chinensis Franch. and Tetradium ruticarpum (A. Juss.) Hartley in the ratio of 6:1 (w/w). In this study, ZJP was evaluated for its gastroprotective potential against mucosal lesions induced by ethanol in mice.
Materials and methods: 50 mice were assigned to 5 groups: groups 1 and 2 were given distilled water orally. Group 3 was administered omeprazole 20 mg/kg, groups 4 and 5 were given ZJP (1 g/kg, 2 g/kg, respectively). After an additional hour, the mice in groups 2-5 received ethanol (0.2 ml/kg) orally while group 1 received distilled water instead. Mice were killed after 4 h and their serum and stomachs subjected to further studies. The superoxidase dismutase (SOD) activities and malondialdehyde (MDA) level in serum were assayed by SOD and MDA kits, respectively. The myeloperoxidase (MPO) activities in stomachs were assayed by MPO kit. The levels of inflammatory mediators including tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and interleukin-1 beta (IL-1 beta) in serum were assayed by enzyme-linked immunosorbent assay method (ELISA). Pathological changes were observed by hematoxylin-eosin (HE) staining. The levels of nuclear factor-kappa Bp65 (NF-kappa Bp65), P-NF-kappa Bp65, P-I kappa B alpha, I kappa B alpha, P-IKK alpha, IKK alpha, P-IKK beta, IKK beta in stomachs were assayed by western blot.
Results: The data showed that treatment with the ZJP markedly attenuated MPO, MDA, TNF-alpha, IL-6, IL-1 beta and increased SOD; and ZJP also decreased protein levels of P-NF-kappa Bp65, P-I kappa B alpha, P-IKK alpha and P-IKK beta in gastric stomachs.
Conclusion: It was concluded that ZJP may represents a potential therapeutic option to reduce the risk of gastric ulceration and the gastroprotective activity of ZJP might contribute in adjusting the inflammatory cytokine by regulating the NF-kappa B signaling pathway.

• 出版日期2015-9-15
• 单位长春大学; 中国药科大学