科研之友
微信
新浪微博
Facebook
分享链接
摘要
Background and ObjectiveThe gingival epithelium is a first line of defense against bacterial challenge. E-cadherin (E-cad) plays an important role in cell-cell adhesion as a barrier in the epithelium. Recently, a decrease in the expression of E-cad has been observed in inflamed gingival tissue. The aims of this study were to clarify the changes in E-cad expression and barrier function in human gingival epithelial cells stimulated with Porphyromonas gingivalis-lipopolysaccharide (P. gingivalis-LPS) and to evaluate the influence of these changes on the inflammatory reaction. Furthermore, to clarify the mechanism of the E-cad changes induced by P. gingivalis-LPS, we focused on reactive oxygen species (ROS) that are reported to induce a decrease in E-cad expression.Material and Methods: Human gingival epithelial cells were incubated in Humedia-KG2 in the presence or absence of P. gingivalis-LPS and antioxidants to analyze ROS involvement in P. gingivalis-LPS-induced E-cad changes. E-cad protein expression was analyzed by immunofluorescence staining. To investigate barrier function and inflammatory changes, we performed transport and cytokine assays using gingival epithelial cells and macrophages co-culture model in transwell plates. Medium containing 10 g/mL P. gingivalis-LPS (transport substance) was added to the upper compartment, which harvested gingival epithelial cells, and medium without P. gingivalis-LPS was added to the lower compartment, which harvested macrophages. In the transport assay, P. gingivalis-LPS penetration was analyzed using the Limulus amebocyte lysate test. In the cytokine assay, we examined the change in tumor necrosis factor- (TNF-) production from the macrophages in the lower compartment using enzyme-linked immunosorbent assay. ResultsExpression of E-cad in human gingival epithelial cells was decreased by P. gingivalis-LPS, and the decrease in E-cad accelerated the penetration of P. gingivalis-LPS through the monolayer. In addition, the concentration of TNF- was higher under the E-cad reduced monolayer. Antioxidants, particularly vitamin E and l-ascorbic acid 2-phosphate magnesium salt, inhibited the decrease in E-cad expression, penetration of P. gingivalis-LPS and increase in TNF-. ConclusionThese results suggest that the decrease in E-cad caused by P. gingivalis-LPS leads to destruction of the epithelial barrier function in human gingival epithelial cells, and finally accelerates the inflammatory reaction under the barrier. Antioxidants, particularly vitamin E and l-ascorbic acid 2-phosphate magnesium salt, may restore the impaired function by scavenging ROS, which are related to the decrease in E-cad expression by P. gingivalis-LPS.
- 出版日期2017-2
