A novel ultrasensitive phosphate amperometric nanobiosensor based on the integration of pyruvate oxidase with highly ordered gold nanowires array

作者：Ogabiela, Edward; Adeloju, Samuel B^*; Cui, Jiewu; Wu, Yucheng; Chen, Wei

来源：Biosensors and Bioelectronics, 2015, 71: 278-285.

DOI：10.1016/j.bios.2015.04.026

• Phosphate
• Pyruvate oxidase
• Gold nanowires array
• Nanobiosensor
• Crosslinking
• Amperometry

A novel phosphate amperometric nanobiosensor, based on an intimate integration of pyruvate oxidase (PyOx) and its cofactors, thiamine pyrophosphate (TPP) and flavin adenine dinucleotide (FAD), with a highly ordered gold nanowires array (AuNWA) has been developed. The successful integration of PyOx and the co-factors, via crosslinking with bovine serum albumin (BSA) and glutaraldehyde (GLA), onto the AuNWA was confirmed by cyclic voltammetry and amperometry. The resulting nanobiosensor achieved a detection limit of 0.1 mu M, a linear concentration range of 12.5-1000 mu M, and a sensitivity of 140.3 mu A mM(-1) cm(-2). Notably, the incorporation of the AuNWA reduced the required PyOx concentration by 70-120 fold and the presence of common interferants, such as chloride, sulfate, fluoride, nitrite and nitrate ions did not interfere with phosphate detection. Furthermore, the nanobiosensor demonstrated a very high stability with repeated use over two weeks and was successfully used for the determination of phosphate in water samples with an average recovery of 96.6 +/- 4.9%.

• 出版日期2015-9-15
• 单位合肥工业大学