A novel subpopulation of monocyte-like cells in the human lung after lipopolysaccharide inhalation

作者:Brittan Mairi*; Barr Laura; Morris Andrew Conway; Duffin Rodger; Rossi Fiona; Johnston Shonna; Monro Graham; Anderson Niall; Rossi Adriano G; McAuley Danny F; Haslett Christopher; Hirani Nik; Dhaliwal Kev; Simpson A John
来源:European Respiratory Journal, 2012, 40(1): 206-214.
DOI:10.1183/09031936.00113811

摘要

The co-ordinated recruitment of monocyte subpopulations, neutrophils and regulatory T-cells (Tregs) during the early stages of human acute lung inflammation remains poorly understood. We therefore performed a detailed characterisation of these lineages in the blood and lungs in a model of human acute lung inflammation. %26lt;br%26gt;Healthy volunteers inhaled lipopolysaccharide (LPS) or saline (n=6 for each group). Blood was collected at 0, 2, 4, 6 and 8 h and bronchoscopy with bronchoalveolar lavage (BAL) performed at 8 h. Multiparameter flow cytometry was used to characterise monocyte subpopulations, neutrophils and Tregs in the blood and lung. %26lt;br%26gt;Inhalation of LPS was associated with significant blood and BAL fluid neutrophilia. Blood populations of monocyte subpopulations and Tregs were unaltered by LPS. In contrast, LPS induced an accumulation of a pulmonary monocyte-like cell (PMLC) population, which was further subdivided into %26quot;inducible%26apos;%26apos; CD14(++)CD16(-) and %26quot;resident%26apos;%26apos; CD14(++)CD16(+) subsets. Inducible PMLCs were significantly increased following LPS inhalation (p=0.0046), whereas resident PMLCs were unchanged. In addition, we noted a significant decrease in Tregs in BAL fluid with LPS inhalation (p=0.027). %26lt;br%26gt;The early stages of LPS-induced inflammation in humans is characterised by pulmonary accumulation of a novel inducible monocyte-like subpopulation, accompanied by significant changes in both neutrophil and Treg numbers.

  • 出版日期2012-7