Novel elongase of Pythium sp with high specificity on Delta(6)-18C desaturated fatty acids

作者：Jeennor Sukanya; Cheawchanlertfa Pattsarun; Suttiwattanakul Sarinya; Panchanawa**** Sarocha; Chutrakul Chanikul; Laoteng Kobkul^*

来源：Biochemical and Biophysical Research Communications, 2014, 450(1): 507-512.

DOI：10.1016/j.bbrc.2014.06.004

• Delta(6)-Fatty acid elongase
• Polyunsaturated fatty acid
• Substrate specificity and preference
• Pythium sp.

We identified a novel elongase gene from a selected strain of the Oomycete, Pythium sp. BCC53698. Using a PCR approach, the cloned gene (PyElo) possessed an open reading frame (ORF) of 834 bp encoding 277 amino acid residues. A similarity search showed that it had homology with the PUFA elongases of several organisms. In addition, the signature characteristics, including four conserved motifs, a histidine-rich catalytic motif and membrane-associated feature were present in the Pythium gene. Heterologous expression in Saccharomyces cerevisiae showed that it was specific for fatty acid substrates, having a double bond at Delta(6)-position, which included gamma-linolenic acid (GLA) and stearidonic acid (STA), and preferentially elongated the n3-18C PUFA. This is an elongase in Oomycete fungi, which displays very high specificity on Delta(6)-18C desaturated fatty acids. This will be a powerful tool to engineer PUFA biosynthesis in organisms of interest through the n-6 series pathway for producing value-added fatty acids.

• 出版日期2014-7-18