Anti-biofouling properties of an amphoteric polymer brush constructed on a glass substrate

作者：Kitano Hiromi^*; Kondo Takuya; Kamada Tomohiro; Iwanaga Shintaroh; Nakamura Makoto; Ohno Kohji

来源：Colloids and Surfaces B: Biointerfaces , 2011, 88(1): 455-462.

DOI：10.1016/j.colsurfb.2011.07.029

• Amphoteric polymer
• Anti-biofouling properties
• Polymer brush
• RAFT polymerization

An amphoteric copolymer brush of methacrylic acid (MA) and 2-(dimethylamino)ethyl methacrylate (DMAEMA) was prepared by reversible addition-fragmentation chain-transfer (RAFT) polymerization using both a free chain transfer agent (n-butylsulfanylthiocarbonylsulfanyl-2-methyl propionic acid) and a radical initiator (4,4'-azobis(4-cyanopentanoic acid)) covalently fixed to a glass substrate. An aqueous solution of the copolymer, Poly(MA-r-DMAEMA), which was simultaneously obtained in liquid phase, had a sufficiently small polydispersity ill its molecular weight. The copolymer brush showed effective suppression of non-specific adsorption of bovine serum albumin and egg white lysozyme to the brush. In contrast, both negatively charged PolyMA and positively charged PolyDMAEMA brushes significantly adsorbed the proteins irrespective of their net charges. Upon ion beam irradiation, furthermore, a hollow space with a designed shape could be made on the glass substrate, and both HEK293 and HepG2 cells non-specifically adhered to the space, forming aggregates, while no adhesion to the non-treated area on the brush was observed. These results suggest that the amphoteric polymer brushes will be useful materials for biomedical applications.

• 出版日期2011-11-1