Antifungal Activity of a Liposomal Itraconazole Formulation in Experimental Aspergillus flavus Keratitis with Endophthalmitis

作者：Goiana Leal Andre Ferraz; Leite Melyna Chaves; Quirino Medeiros Caroline Sanuzi; Ferro Cavalcanti Isabella Macario; Wanderley Almir Goncalves; Santos Magalhaes Nereide Stela; Neves Rejane Pereira

来源：Mycopathologia, 2015, 179(3-4): 225-229.

DOI：10.1007/s11046-014-9837-2

• Liposomal itraconazole
• Antifungal activity
• Keratitis with endophthalmitis
• Aspergillus flavus

The aim of this study was to assess the efficacy of topical application of a liposomal formulation of itraconazole for the treatment of experimental keratitis with endophthalmitis caused by Aspergillus flavus. The liposomes were obtained by the lipid film hydration method followed by sonication. Adult female Wistar rats (weighing 200-220 g) were immunosuppressed by intraperitoneal injection of 150 mg/kg of cyclophosphamide 3 days before infection by exposure to the fungus A. flavus (10(7) spores/ml). Forty-eight hours later, the animals were treated with the liposomal formulation. For comparison, one group of animals (n = 6) was treated with the same drug not encapsulated. At the end of the experiment, the animals were evaluated for clinical signs and number of colony forming units (CFU/g), along with direct microscopic examination. The results indicated that the liposomal formulation of itraconazole has better antifungal activity than the unencapsulated drug in the treatment of fungal keratitis with endophthalmitis caused experimentally by A. flavus in Wistar rats.

• 出版日期2015-4