Ultrasonic pretreatment in lipase-catalyzed synthesis of structured lipids with high 1,3-dioleoyl-2-palmitoylglycerol content

作者：Liu, Si-lei; Dong, Xu-yan^*; Wei, Fang; Wang, Xiang; Lv, Xin; Zhong, Juan; Wu, Lin; Quek, Siew-young; Chen, Hong

来源：Ultrasonics Sonochemistry, 2015, 23: 100-108.

DOI：10.1016/j.ultsonch.2014.10.015

• Ultrasonic pretreatment
• 1,3-Dioleoyl-2-palmitoylglycerol
• Lipase-catalyzed
• Acidolysis
• Structured lipid
• Acyl migration

Production of structured lipid 1,3-dioleoyl-2-palmitoylglycerol (OPO), from tripalmitin (PPP) and oleic acid (OA) using lipases and ultrasonic pretreatment was conducted. Factors influencing both the ultrasonic conditions and enzymatic reaction were investigated. Optimum conditions could be attained with 6 min pretreatment time, 50% ultrasonic power, 3 s/9 s (work/pause) cycle of ultrasonic pulse, 1:8 PPP/OA molar ratio, 12% enzyme dosage and 50 degrees C temperature of. At the optimum conditions, the OPO yield of 51.8% could be achieved in 4 h. Studies showed that the OPO content increased to 35.9% in 1 h with ultrasonic pretreatment, in comparison to 4 h without ultrasonic pretreatment. Reuse of Lipozyme RM IM for 10 cycles under ultrasonic irradiation did not cause essential damage to its lipase activity. Reaction kinetic model fitted well with the proposed Ping-Pong mechanism. The apparent kinetic constant (V-m'/K-2) of ultrasound pretreatment reaction was 2.52 times higher than the conventional mechanical stirring, indicating that ultrasound pretreatment enhanced the substrates affinity to the enzyme. This study confirmed that ultrasonic pretreatment was more efficient in OPO production than conventional mechanical agitation.

• 出版日期2015-3
• 单位中国农业科学院油料作物研究所