Antimicrobial susceptibility of Helicobacter pylori strains isolated in Colombia

作者：Alvarez Adalucy^*; Ignacio Moncayo Jose; Javier Santacruz Jorge; Fernanda Corredor Luisa; Reinosa Elizabeth; William Martinez Jose; Beltran Leonardo

来源：Revista Medica de Chile, 2009, 137(10): 1309-1314.

• Antibacterial agents
• Helicobacter pylori
• Metronidazole

Background: Helicobacter pylori antimicrobial resistance rates differ among countries and even between different areas of a country. In Colombia, the most commonly used antimicrobials for the treatment of H pylori infection are amoxicillin, clarithromycin and metronidazole. Aim: To determine antimicrobial susceptibility of H pylori strains isolated in Colombia. Materials and methods: Eighty eight strains of H pylori were isolated and identified by microbiological methods and confirmed with polymerase chain reaction (PCR). The detection of antimicrobial resistance to amoxicillin, clarithromycin metronidazole and tetraclycline, was conducted by the Etest method. Mutations in the 23S rDNA, involved in resistance to clarithromycin, were detected using PCR and restriction fragment length polymorphism. Results: Eighty eight and 2.2% of the strains were resistant to metronidazole and clarithromycin, respectively. No isolate was simultaneously resistant to amoxicillin or tetracycline. The two clarithromycin resistant strains were homozygous for the A2143G mutation. No mutations were found in the remaining 86 susceptible strains. Conclusions: The high rate of metronidazole resistance in our population precludes the use of this drug for the empirical treatment of H pylori infection (Rev Med Chile 2009; 137: 1309-14).

• 出版日期2009-10