Diverse T-cell responses characterize the different manifestations of cutaneous graft-versus-host disease

作者：Brueggen Marie Charlotte; Klein Irene; Greinix Hildegard; Bauer Wolfgang; Kuzmina Zoya; Rabitsch Werner; Kalhs Peter; Petzelbauer Peter; Knobler Robert; Stingl Georg; Stary Georg^*

来源：Blood, 2014, 123(2): 290-299.

DOI：10.1182/blood-2013-07-514372

Graft-versus-host disease (GVHD) is a major complication of allogeneic hematopoietic stem cell transplantation (HCT) and can present in an acute (aGVHD), a chronic lichenoid (cIGVHD), and a chronic sclerotic form (csGVHD). It is unclear whether similar or different pathomechanisms lead to these distinct clinical presentations. To address this issue, we collected lesional skin biopsies from aGVHD (n = 25), cIGVHD (n = 17), and csGVHD (n = 7) patients as well as serial nonlesional biopsies from HCT recipients (prior to or post-HCT) (n = 14) and subjected them to phenotypic and functional analyses. Our results revealed striking differences between aGVHD and clGVHD. In aGVHD, we found a clear predominance of T helper (Th)2 cytokines/chemokines and, surprisingly, of interleukin (IL)-22 messenger RNA as well as an increase of IL-22 producing CD4(+) T cells. Thymic stromal lymphopoietin, a cytokine skewing the immune response toward a Th2 direction, was elevated at day 20 to 30 post-HCT in the skin of patients who later developed aGVHD. In sharp contrast to aGVHD, the immune response occurring in clGVHD showed a mixed Th1f Th17 signature with upregulated Th1f Th17 cytokine/chemoldne transcripts and elevated numbers of interferon-gamma- and IL-17 producing CD8(+) T cells. Our findings shed new light on the T-cell responses involved in the different manifestations of cutaneous GVHD and identify molecular signatures indicating the development of the disease.

• 出版日期2014-1-9