Applications of C-14-AMS in biomedical sciences (Bio-C-14-AMS)

作者：Wang HF; Sun HF; Liu YF^*

来源：Chinese Science Bulletin, 2001, 46(7): 537-543.

DOI：10.1007/BF02900404

• accelerator mass spectrometry (AMS)
• DNA adduction
• protein adduction
• biomarker for carcinogenesis
• tracer of xenobiotics

Accelerator mass spectrometry (AMS) is an ultrasensitive measure for tracing C-14 labeled molecules in vivo or detecting the biomarker for assessment of carcinogenesis. In this review, basic principles, wide applications and new progresses of C-14-bio-AMS are presented. It has been a new advanced tool for measuring the adduction of biological molecules with xenobiotics, including carcinogens, drugs, agrochemicals, nicotine, etc. The successful applications have proven the effectiveness of AMS to assessing cancer risk, screening drug toxicity and studying nutrients uptake. bn particular, AMS is characterized by measuring xenobiotics at very low dose levels relevant to human environmental exposure. It is sensitive and precise to an attomole (10(-18) mole) or less of C-14 per mg carbon. Although it has some shortcomings, undoubtedly, AMS possesses an evident merit of high sensitivity and will have widespread applications in the biomedical sciences.

• 出版日期2001-4
• 单位北京大学