A Comprehensive Map of the Human Urinary Proteome

作者：Marimuthu Arivusudar; O'Meally Robert N; Chaerkady Raghothama; Subbannayya Yashwanth; Nanjappa Vishalakshi; Kumar Praveen; Kelkar Dhanashree S; Pinto Sneha M; Sharma Rakesh; Renuse Santosh; Goel Renu; Christopher Rita; Delanghe Bernard; Cole Robert N; Harsha H C; Pandey Akhilesh^*

来源：Journal of Proteome Research, 2011, 10(6): 2734-2743.

DOI：10.1021/pr2003038

• body fluids
• biomarkers
• glycoproteins

The study of the human urinary proteome has the potential to offer significant insights into normal physiology as well as disease pathology. The information obtained from such studies could be applied to the diagnosis of various diseases. The high sensitivity, resolution, and mass accuracy of the latest generation of mass spectrometers provides an opportunity to accurately catalog the proteins present in human urine, including those present at low levels. To this end, we carried out a comprehensive analysis of human urinary proteome from healthy individuals using high-resolution Fourier transform mass spectrometry. Importantly, we used the Orbitrap for detecting ions in both MS (resolution 60 000) and MS/MS (resolution 15 000) modes. To increase the depth of our analysis we characterized both unfractionated as well as lectin-enriched proteins in our experiments. In all, we identified 1823 proteins with less than 1% false discovery rate, of which 671 proteins have not previously been reported as constituents of human urine. This data set should serve as a comprehensive reference list for future studies aimed at identification and characterization of urinary biomarkers for various diseases.

• 出版日期2011-6