Ultrasound-mediated drug delivery using liposomes modified with a thermosensitive polymer

作者：Ninomiya Kazuaki; Kawabata Shinya; Tashita Hiroyuki; Shimizu Nobuaki^*

来源：Ultrasonics Sonochemistry, 2014, 21(1): 310-316.

DOI：10.1016/j.ultsonch.2013.07.014

• Drug delivery
• Temperature-sensitive liposomes
• Thermosensitive polymer
• Ultrasound
• Cavitation

Ultrasound-mediated drug delivery was established using liposomes that were modified with the thermosensitive polymer (TSP) poly(NIPMAM-co-NIPAM), which sensitized the liposomes to high temperatures. TSP-modified liposomes (TSP liposomes) released encapsulated calcein under 1 MHz ultrasound irradiation at 0.5 W/cm(2) for 120 s as well as the case under incubation at 42 degrees C for 15 min. In addition, uptake of the drug released from TSP liposomes by cancer cells was enhanced by ultrasound irradiation. In a cell injury assay using doxorubicin (DOX)-loaded TSP liposomes and ultrasound irradiation, cell viability of HepG2 cells at 6 h after ultrasound irradiation (1 MHz, 0.5 W/cm(2) for 30 s) with DOX-loaded TSP liposomes (TSP/Iipid ratio = 1) was 60%, which was significantly lower than that of the control conditions such as DOX-loaded TSP liposomes alone and DOX-loaded intact liposomes under ultrasound irradiation.

• 出版日期2014-1