Monitoring and control of inertial cavitation activity for enhancing ultrasound transfection: The SonInCaRe project

作者：Inserra C^*; Labelle P; Loughian C Der; Lee J L; Fouqueray M; Ngo J; Poizat A; Desjouy C; Munteanu B; Lo C W; Vanbelle C; Rieu J P; Chen W S; Bera J C

来源：IRBM, 2014, 35(2): 94-99.

DOI：10.1016/j.irbm.2014.02.010

Sonoporation process, at the origin of ultrasound cell transfection, is ruled by the interaction between cells and cavitating bubbles. Due to the stochastic behavior of acoustic cavitation, there exists a need in ensuring a stable state of cavitation within a medium during cell transfection to enhance transfection efficiency and control mortality. The goal of the SonInCaRe project is thus to define a controlled-cavitation device in order to monitor, control and stabilize inertial cavitation activity during cell sonication in real-time. This device, based on a feedback loop acting in real-time, allows ensuring fixed cavitation conditions during a pulsed sonication. Its application to suspended and adherent cells transfection shows better reproducibility compared to the fixed acoustic intensity sonication. The regulation device thus provides a better control of cavitation activity and its bioeffects which are of crucial importance for clinical applications of ultrasound-mediated gene transfection.

• 出版日期2014-4