Highly efficient and facile fabrication of monodispersed Au nanoparticles using pullulan and their application as anticancer drug carriers

作者：Laksee Sakchai; Puthong Songchan; Teerawatananond Thapong; Palaga Tanapat; Muangsina Nongnuj^*

来源：Carbohydrate Polymers, 2017, 173: 178-191.

DOI：10.1016/j.carbpol.2017.05.101

• Gold nanoparticles
• Pullulan
• Efficient fabrication
• Cytotoxicity
• KATO-III cells
• Cassiarin a chloride derivatives

This work presented a simple, rapid, green and efficient approach to the synthesis of gold nanoparticles using pullulan as a reducing/stabilizing/capping agent for drug delivery systems to increase the safety and efficacy of these systems. Monodispersed AuNP5@pullulan with prolonged stability were fully characterized by UV-VIS, FTIR, TEM, EDX, TGA and zeta potential analyses. A mechanism of AuNP5 formation was proposed in which pullulan created reducing species for the reduction of Au3+ to AuNP5 (Au-0) that resulted in the formation of spherical AuNP5@pullulan with an average size of approximately 11 +/- 5 nm, while the hydroxyl groups of pullulan were oxidized to carboxylate compounds. Novel cassiarin A chloride derivatives (3d and 3i) as candidate anticancer drugs were successfully loaded onto AuNP5@pullulan through electrostatic interactions. AuNP5@pullulan-3d (IC50 = 6.0 +/- 0.1 mu M) and AuNP5@pullulan-3i (5.2 +/- 0.1 mu M) exhibited a 10.2-fold and 7.1-fold higher cytotoxicity against KATO-III cells than free compounds 3d (60.9 +/- 0.6 mu M), 3i (37.1 +/- 0.2 mu M) and cisplatin (64.5 +/- 0.9 mu M), respectively. AuNP5@pullulan exhibited high cellular uptake, biocompatibility and non-cytotoxicity to normal cells. Therefore, AuNP5@pullulan-3d or AuNP5@pullulan-3i have the potential to be developed for treatment of gastric cancer.

• 出版日期2017-10-1