A sensitive aptasensor based on molybdenum carbide nanotubes and label-free aptamer for detection of bisphenol A

作者：He, Meng-Qi; Wang, Kun; Wang, Jin; Yu, Yong-Liang^*; He, Rong-Huan^*

来源：Analytical and Bioanalytical Chemistry, 2017, 409(7): 1797-1803.

DOI：10.1007/s00216-016-0123-7

• Biosensor
• Aptamer
• Label-free
• Mo2C nanotubes
• Bisphenol A

To specifically and sensitively identify bisphenol A (BPA) with a simple and rapid method is very important for food safety. Using an anti-BPA aptamer and Mo2C nanotubes, we developed a label-free and low-background signal biosensor for BPA detection. The anti-BPA aptamer drastically increased the fluorescence signal of N-methylmesoporphyrin IX under an assistance of Help-DNA. Additionally, BPA can interact with the anti-BPA aptamer and switch its conformation to prevent the formation of a G-quadruplex, resulting in fluorescence quenching. Simultaneously, Mo2C nanotubes can reduce the background signals due to the adsorption of Help-DNA on their surface. This method shows a linear range of 2-20 nM with a detection limit of 2 nM for detecting BPA. This label-free BPA aptasensor with low background signal is inexpensive, easy to use, and can be applied to determine BPA in real water samples.

• 出版日期2017-3
• 单位东北大学