Hsa_circ_0071589 promotes carcinogenesis via the miR-600/EZH2 axis in colorectal cancer

作者：Wang Yong; Xuan Zhuoqi; Wang Baocheng; Zhang Dongsheng; Zhang Chuan; Sun Yueming^*

来源：Biomedicine & Pharmacotherapy, 2018, 102: 1188-1194.

DOI：10.1016/j.biopha.2018.03.085

• Colorectal cancer
• hsa_circ_0071589
• miR-600
• EZH2
• Proliferation
• Invasion
• Migration

Previous studies have revealed that miRNAs and lncRNAs participate in the pathogenesis of colorectal cancer (CRC); however, whether circular RNAs (circRNAs) are also involved remains unclear. In the present study, qRTPCR was used to examine the expression of hsa_circ_0071589, miR-600, and enhancer of zeste homolog 2 (EZH2) in CRC. MTT assay, colony formation assay, transwell assay, and wound-healing assay were performed to assess the effects of hsa_circ_0071589, miR-600, and EZH2 on CRC cell viability, proliferation, invasion, and migration. Bioinformatics analysis, luciferase reporter assay, and RIP assay were used to explore the correlations among hsa_circ_0071589, miR-600, and EZH2 expression in CRC cells. The results showed that hsa_circ_0071589 expression was significantly higher in CRC tissues than in normal tissues. Blockage of hsa_circ_0071589 in CRC cells inhibited tumor growth, invasion and migration. Hsa_circ_0071589 was able to promote the expression of EZH2 by acting as a sponge of miR-600. In addition, miR-600 expression was negatively correlated to hsa_circ_0071589 expression in CRC tissues. These results demonstrated that the hsa_circ_0071589/miR-600/EZH2 axis may play critical regulatory roles in the pathogenesis of CRC and may serve as a novel therapy target in CRC.

• 出版日期2018-6
• 单位南京医科大学