NF kappa B-dependent increase of kynurenine pathway activity in human placenta: Inhibition by sulfasalazine

作者：Dharane P; Manuelpillai U; Wallace E; Walker D W^*

来源：Placenta, 2010, 31(11): 997-1002.

DOI：10.1016/j.placenta.2010.09.002

• Kynurenines
• Sulfasalazine
• Inflammation
• Placenta
• NF kappa B

Catabolism of tryptophan via the kynurenine pathway is up-regulated in the human placenta by infection, resulting in the release of pro-inflammatory and neuroactive metabolites into the fetal circulation. In this study we determined if activation of NF kappa B is involved in the inflammation-induced increase of kynurenine pathway activity in the human placenta. Placentae obtained after elective caesarian section at 37-40 weeks gestation (n = 8), and explants (35-40 mg) prepared from terminal villi were incubated under standard conditions in the presence of 10 mg/ml LPS for 24 or 48 h; duplicates of each explant were incubated either with or without 5 mM sulfasalazine added to the medium. Expression of rnRNAs for key kynurenine-forming enzymes, indoleamine 2,3-dioxygrenase (IDO) and tryptophan 2,3-doalxygenase (TDO) and the inflammatory cytokines TNF alpha and 16 was studied by RT-PCR. Kynurenine output by explants was measured in samples in the incubation medium by absorbance at 363 nm after separation from other metabolites using an HPLC technique. Expression of IDO, TDO, TNF alpha and IL6 mRNAs was increased with LPS treatment, a response mitigated by the presence of sulfasalazine (P < 0.01, P < 0.01, P = 0.03 & P = 0.04). Kynurenine output into the culture medium increased with LPS treatment but this was also prevented by sulfasalazine at 24 h (mean +/- SEM; 412.1 +/- 40 vs. 147.7 +/- 48.9 nM/mg, P = 0.01) and 48 h (636 +/- 39.1 vs. 135.5 +/- 29.8 nM/mg, P = 0.001, respectively). Sulfasalazine inhibited the LPS induction of both the kynurenine pathway and pro-inflammatory cytokines in the placenta, implicating NF kappa B in the LPS effect. Direct measurement of NF kappa B activity showed that sulfasalazine decreased NF kappa B activation under both control and LPS-treated conditions. These observations show that kynurenine pathway activity in the human placenta is increased by a NF kappa B dependent pathway, and suggests a new therapeutic strategy for the management of pregnancies with in utero infection.

• 出版日期2010-11
• 单位河北医科大学