Endoplasmic reticulum stress-induced apoptosis in the penumbra aggravates secondary damage in rats with traumatic brain injury

作者：Sun, Guo-zhu^*; Gao, Fen-fei; Zhao, Zong-mao; Sun, Hai; Xu, Wei; Wu, Li-wei; He, Yong-chang

来源：Neural Regeneration Research, 2016, 11(8): 1260-1266.

DOI：10.4103/1673-5374.189190

• nerve regeneration
• endoplasmic reticulum stress
• apoptosis
• caspase-12
• caspase-3
• traumatic penumbra
• traumatic brain injury
• neural regeneration

Neuronal apoptosis is mediated by intrinsic and extrinsic signaling pathways such as the membrane-mediated, mitochondrial, and endoplasmic reticulum stress pathways. Few studies have examined the endoplasmic reticulum-mediated apoptosis pathway in the penumbra after traumatic brain injury, and it remains unclear whether endoplasmic reticulum stress can activate the caspase-12-dependent apoptotic pathway in the traumatic penumbra. Here, we established rat models of fluid percussion-induced traumatic brain injury and found that protein expression of caspase-12, caspase-3 and the endoplasmic reticulum stress marker 78 kDa glucose-regulated protein increased in the traumatic penumbra 6 hours after injury and peaked at 24 hours. Furthermore, numbers of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling-positive cells in the traumatic penumbra also reached peak levels 24 hours after injury. These findings suggest that caspase-12-mediated endoplasmic reticulum-related apoptosis is activated in the traumatic penumbra, and may play an important role in the pathophysiology of secondary brain injury.

• 出版日期2016-8
• 单位河北医科大学; 汕头大学