A fusion protein N-cadherin-Fc as an artificial extracellular matrix surface for maintenance of stem cell features

作者：Yue Xiao Shan; Murakami Yuta; Tamai Toshiyuki; Nagaoka Masato; Cho Chong Su; Ito Yoshihiro; Akaike Toshihiro^*

来源：Biomaterials, 2010, 31(20): 5287-5296.

DOI：10.1016/j.biomaterials.2010.03.035

• Adhesion molecule
• N-cadherin
• Extracellular matrix
• Neural cell
• Stem cell

N-cadherin is a cell cell adhesion molecule and plays important roles in neural development. With conventionally used extracellular matrices (ECMs), maintenance of undifferentiated state of stem cells and regulation of their neural differentiation process is very difficult due to the colony formation through intercellular interactions. To overcome the above-mentioned problems, we developed a new artificial ECM to mimic N-cadherin-mediated cell adhesion. In this study, we constructed a chimeric protein (N-cadherin fused to IgG-Fc, abbreviated as N-cad-Fc), which contains extracellular domain of N-cadherin and Fc domain of IgG. We confirmed that N-cad-Fc can stably adsorb to hydrophobic surface. We checked maintenance of undifferentiated state and neural differentiation ability of stem cells cultured on N-cad-Fc-coated surface. Both P19 and MEB5 cells cultured on N-cad-Fc-coated surface showed scattering morphologies without colony formation and higher proliferating potency than conventional culture systems with maintenance of undifferentiated state. Both of two cell lines cultured on N-cad-Fc-coated surface differentiated into neural cells at a single cell level when induced with proper conditions. Furthermore, the expression of neuron-related gene Neurog1 in two cell lines cultured on N-cad-Fc-coated surface was promoted. Therefore, it will be expected that the constructed N-cad-Fc can be used as an artificial ECM for stem cells.

• 出版日期2010-7