Acetylene plasma polymerized surfaces for covalent immobilization of dense bioactive protein monolayers

作者：Yin Yongbai^*; Bilek Marcela M M; McKenzie David R; Nosworthy Neil J; Kondyurin Alexey; Youssef Hani; Byrom Michael J; Yang Wengrong

来源：Surface and Coatings Technology, 2009, 203(10-11): 1310-1316.

DOI：10.1016/j.surfcoat.2008.10.035

• Plasma polymerization
• Protein immobilization
• Covalent attachment
• Quartz crystal microbalance
• FTIR
• Ellipsometer

Smooth polymerized surfaces, suitable for biochemical and biomedical applications, were deposited using a modified plasma enhanced chemical vapour deposition method with acetylene as a reaction precursor. Horseradish peroxidase (HRP) activity assays showed that the protein immobilized on the plasma polymerized surfaces maintained its biological function for a much longer period of time compared to that on uncoated surfaces. The kinetics of HRP attachment to the plasma polymerized surfaces were analyzed using quartz crystal microbalance with dissipation analysis. Spectroscopic ellipsometry and attenuated total reflection Fourier transform infrared spectroscopy were used to determine the thickness and the quantity of the attached protein. The results showed that the plasma polymerized surfaces provided a high density of attachment sites to covalently immobilize a dense monolayer of proteins.

• 出版日期2009-2-25