Description of Comamonas sediminis sp nov., isolated from lagoon sediments

作者：Subhash Y; Bang John J; You Taek H; Lee Sang Seob^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2016, 66(7): 2735-2739.

DOI：10.1099/ijsem.0.001115

Strain S3(T) was isolated from lagoon sediments, and appeared as transparent colonies on agar plates, with cells staining Gram-negative. Catalase and oxidase were positive. S3(T) hydrolyzed starch, casein and tween-20, while urea, chitin, gelatin and tween-80 were not hydrolysed. C-18:1 omega 6C/C-18:1 omega 7C, C-16:1 omega 6C/C-16:1 omega 7c,C-17:0 cyclo and C-16:0 were the predominant fatty acids with minor amounts of C-10:0 3-OH, C-12:0, C-14:0 and C-16:0 2-OH. S3(T) contained diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE) as major polar lipids with minor amounts of unidentified phospholipid (PL) and unidentified lipids (L1-2). Genomic DNA G+C content was 68.3 mol%. 16S rRNA gene sequence comparisons indicated that S3(T) represents a member of the genus Comamonas in family Comamonadaceae of the class Betaproteobacteria. S3(T) has a sequence similarity of 98.96% with Comamonas koreensis YH12(T), 97.93% with Comamonas guangdongensis CY01(T) and <96.97% with other members of the genus Comamonas. DNA DNA hybridization values between S3(T) and the type strains of the most closely related species were clearly below the 70% threshold. On the basis of phenotypic, genotypic and phylogenetic analysis, it is proposed that S3(T) represents a novel species of the genus Comamonas, for which the name Comamonas sediminis sp. nov. is proposed. The type strain is S3(T) (=KEMB 563-466(T) =JCM 31169(T)).

• 出版日期2016-7