An extension of the coconut cream agar method to screen Penicillium citrinum isolates for citrinin production

作者：Mohamed S^*; Flint S; Palmer J; Fletcher G C; Pitt J I

来源：Letters in Applied Microbiology, 2013, 57(3): 214-219.

DOI：10.1111/lam.12102

• citrinin
• coconut cream agar
• fluorescence
• Penicillium citrinum
• screening

A simple and rapid screening method was developed for the detection of citrinin in fungal cultures using Coconut Cream Agar (CCA) described previously for detecting aflatoxin and ochratoxin A. Fifteen isolates of Penicillium citrinum were inoculated onto CCA and incubated at 25 and 30 degrees C for 10days. All isolates produced a distinct yellow green fluorescence on CCA when the reverse side of the agar plates were viewed under long wavelength UV light. Detection was optimal at 25 degrees C after four to 5days of incubation. Isolates positive by the CCA method also tested positive for citrinin production by the TLC agar plug method after growth on CCA, Czapek yeast extract agar and yeast extract sucrose agar. Control cultures were negative by both methods, indicating that the CCA Petri dish method was suitable for screening cultures for citrinin production.

• 出版日期2013-9