Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method

作者：Groves Thomas R; Wang Jing; Boerma Marjan; Allen Antino R^*

来源：Jove-Journal of Visualized Experiments, 2017, (124): e55696.

DOI：10.3791/55696

• Neuroscience
• Issue 124
• Golgi
• hippocampus
• dendritic spines
• dendritic morphology
• complexity
• arborization

Dendritic spines are the protuberances from the neuronal dendritic shafts that contain excitatory synapses. The morphological and branching variations of the neuronal dendrites within the hippocampus are implicated in cognition and memory formation. There are several approaches to Golgi staining, all of which have been useful for determining the morphological characteristics of dendritic arbors and produce a clear background. The present Golgi-Cox method, (a slight variation of the protocol that is provided with a commercial Golgi staining kit), was designed to assess how a relatively low dose of the chemotherapeutic drug 5-flurouracil (5-Fu) would affect dendritic morphology, the number of spines, and the complexity of arborization within the hippocampus. The 5-Fu significantly modulated the dendritic complexity and decreased the spine density throughout the hippocampus in a region-specific manner. The data presented show that the Golgi staining method effectively stained the mature neurons in the CA1, the CA3, and the dentate gyrus (DG) of the hippocampus. This protocol reports the details for each step so that other researchers can reliably stain tissue throughout the brain with high quality results and minimal troubleshooting.

• 出版日期2017-6