A cold-adapted and glucose-stimulated type II alpha-glucosidase from a deep-sea bacterium Pseudoalteromonas sp K8

作者：Li, Wei; Xue, Yi; Li, Jingjing; Yuan, Jing; Wang, Xiaotang; Fang, Wei; Fang, Zemin^*; Xiao, Yazhong

来源：Biotechnology Letters, 2016, 38(2): 345-349.

DOI：10.1007/s10529-015-1987-x

• Cold adapted enzyme
• Genome missing
• Glucose stimulated enzyme
• alpha-Glucosidase
• Pseudoaltemonas

To express and characterize a putative alpha-glucosidase, Pagl, from Pseudoalteromonas sp. K8 obtained via genome mining approach.
Pagl was expressed and purified to homogeneity, with a molecular mass of 60 kDa. It was optimally active at pH 8.5 and 30 A degrees C, and showed cold-adapted activity. Pagl exhibited specific activity towards substrates with alpha-1,4-linkage, with the highest specific activity of 19.4 U/mg for maltose, followed by pNP alpha G and maltodextrins, suggesting that Pagl belongs to the type II alpha-glucosidase. Interestingly, the activity of Pagl is significantly enhanced (2.7 times) in the presence of 200 mM glucose.
The unique catalytic properties of Pagl make it an attractive candidate for several industrial applications.

• 出版日期2016-2
• 单位安徽大学