DETECTION OF DNA ABERRATIONS IN HUMAN CANCERS BY SINGLE-STRAND CONFORMATION POLYMORPHISM ANALYSIS OF POLYMERASE CHAIN-REACTION PRODUCTS

作者：MURAKAMI Y; SUZUKI Y; KISHIMOTO Y; HIROHASHI S; HAYASHI K; SEKIYA T

来源：Tohoku Journal of Experimental Medicine, 1992, 168(2): 247-255.

DOI：10.1620/tjem.168.247

• SSCP ANALYSIS
• LUNG CARCINOMA
• HEPATOCELLULAR CARCINOMA
• RAS GENE
• P53-GENE
• RB GENE

We have developed a simple, sensitive method, single-strand conformation polymorphism (SSCP) analysis, to detect a single nucleotide substitution in a DNA fragment amplified and labeled by the polymerase chain reaction (PCR). Mobility shift of single-stranded DNAs due to their specific conformations on non-denaturing polyacrylamide gel electrophoresis can reveal DNA aberrations. By the PCR-SSCP analysis of DNAs from surgical specimens of human cancers, mutated ras genes (17%) and aberrations of tumor suppressor p53 gene (53%) including loss of one of the two alleles and a mutation in the remaining allele were detected in lung carcinomas and aberrations of both of the p53 and retinoblastoma (RB) genes were detected exclusively in advanced hepatocellular carcinomas.

• 出版日期1992-10