Design of a Ribosyltriazole-Annulated Cyclooctyne for Oligonucleotide Labeling by Strain-Promoted Alkyne-Azide Cycloaddition

作者：van Delft Pieter; de Witte Wilbert; Meeuwenoord Nico J; van Noort Gerbrand J van der Heden; Versluis Frank; Olsthoorn Rene C L; Overkleeft Herman S; van der Marel Gijs A^*; Filippov Dmitri V

来源：European Journal of Organic Chemistry, 2014, 2014(34): 7566-7571.

DOI：10.1002/ejoc.201403086

• Solid-phase synthesis
• Oligonucleotides
• Click chemistry
• Conjugation
• Dyes/Pigments

A ribosyltriazole ring-fused cyclooctyne was prepared and converted into the corresponding phosphoramidite, which was applied in the automated synthesis of DNA and RNA oligomers. Ensuing strain-promoted alkyne-azide cycloaddition of the obtained oligonucleotides to fluorescent azides yielded the corresponding fluorescent oligonucleotide conjugates.

• 出版日期2014-12