Anti-tumour Effects of Exosomes in Combination with Cyclophosphamide and Polyinosinic-Polycytidylic Acid

作者：Guo F; Chang C K^*; Fan H H; Nie X X; Ren Y N; Liu Y Y; Zhao L H

来源：Journal of International Medical Research, 2008, 36(6): 1342-1353.

DOI：10.1177/147323000803600623

• CYCLOPHOSPHAMIDE
• POLY I:C
• ANTI-CANCER AGENTS
• CANCER
• TUMOUR IMMUNOLOGY
• REGULATORY T CELLS
• EXOSOMES
• TOLL-LIKE RECEPTOR

We examined the anti-tumour activity of exosomes derived from dendritic cells (DCs) in combination with cyclophosphamide (CTX) and polyinosinic-polycytidylic acid sodium salt (poly I:C). DCs were pulsed with L1210 lymphocytic leukaemia cell antigen and lipopolysaccharide. The exosomes that the DCs secreted were purified. In vitro, the anti-tumour activity of exosomes was assessed by measuring their ability to induce spleen cell proliferation and the extent to which they induced spleen cells to kill L1210 cells. Poly I:C was able to induce DC maturation. DC-derived exosomes stimulated spleen cell proliferation and enhanced the cytotoxic effects of spleen cells in vitro. DC-derived exosomes, in combination with CTX and poly 1:C, suppressed L1210 tumour growth in vivo and gave the greatest prolongation of survival time in tumour-bearing DBA2 mice. These findings suggest that this combination of a tumour vaccine, a conventional anti-cancer agent and a promoter of DC maturation might be a useful anti-cancer therapy.

• 出版日期2008-12
• 单位上海市血液中心; 苏州大学