Molecular analysis of duck enteritis virus US3, US4, and US5 gene

作者：Zhao Yan; Wang Jun Wei^*; Ma Bo; Liu Fengyuan

来源：Virus Genes, 2009, 38(2): 289-294.

DOI：10.1007/s11262-008-0326-x

• Duck enteritis virus
• US3
• US4
• US5
• Single oligonucleotide nested polymerase chain reaction

Here, we first present unique short (US)3, US4, and US5 gene sequences, with analysis, of duck enteritis virus (DEV) vaccine strain C-KCE. The assembled sequence comprises 5,742 nucleotides, which are amplified from the DEV genome by single oligonucleotide-nested polymerase chain reaction with primers designed according to our previous acquired sequence deposited in GenBank (accession no. EF619046). The predicted gene arrangement is colinear with the alphaherpesvirus herpes simplex virus within the US region. The N-glycosylated sites, signal peptide, transmembrane helices, RNA polymerase II transcriptional control elements, and polyadenylation signal, were predicted with network prediction programs. Phylogenetic analysis of the three putative proteins revealed that they had a close evolutionary relationship with the subfamily of Alphaherpesvirinae.

• 出版日期2009-4
• 单位东北农业大学